Apparent digestibility coefficients for protein and carbohydrates in the foregut (Segment 1, Figures 6, 7) were higher (p < 0.05, glmPQL) in the fish fed the dry compared to moist diets. However, in the midgut (Segments 2 and 3) and hindgut (Segment 4), the ADC for proteins and carbohydrates were not significantly different between fish fed dry or moist diets. 05, Figure 8). However, the ADC for fatty acids were different between three fatty acid classes. Polyunsaturated fatty acids (PUFA) had higher ADCs in all the four segments than monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) (wilcox.test, p < 0.01). The ADC for SFA was lower than that for MUFA in Segments 1 and 3 (wilcox.test, p < 0.05); but they were similar in Segments 2 and 4. ADCs showed that digestion and absorption of protein, carbohydrate and lipid had similar patterns in the intestine of ballan wrasse. Up to , , and % of carbohydrates, proteins, and lipid, respectively, were digested and absorbed in Segment 1. In addition, the ADC of all macronutrients increased significantly along the intestine, with the ADCs increasing to a maximum for carbohydrate and lipid in Segment 3, and for protein in Segment 4 (glmPQL, p < 0.05, Figures 6–8).
Brand new digestibility of lipid was not influenced by diet dampness peak (glmPQL, p > 0
Figure 6. Apparent digestibility coefficient (ADC) of protein in ballan wrasse intestine. The figure shows the ADC (%) of protein in the 4 intestinal segments (S1–S4) of fish fed with dry or moist diets. Asterisk’s ( ? ) above horizontal square brackets indicate significant differences (glmmPQL test in R) between treatments (black line) or between adjacent intestinal segments (gray lines). ??? p < 0.001, ?? p < 0.01, ? p < 0.05. Data are mean ± SEM, n = 6.
Figure 7. Apparent digestibility coefficient (ADC) of carbohydrate in ballan wrasse intestine. The figure shows the ADC (%) of carbohydrate in the 4 intestinal segments (S1–S4) of ballan wrasse fed with dry or moist diets. Asterisk’s ( ? ) above horizontal square brackets indicate significant differences (glmmPQL test in R) between treatments (black line) or between adjacent intestinal segments (gray lines). ??? p < 0.001, ?? p < 0.01. Data are mean ± SEM, n = 6.
Figure 8. Apparent digestibility coefficient (ADC) of lipid in ballan wrasse intestine. The figures show ADC (%) of lipid (total fatty acids), TFA; monounsaturated fatty acids, MUFA; polyunsaturated fatty acids, PUFA; and saturated fatty acids, SFA in the 4 intestinal segments (S1–S4) of ballan wrasse fed dry or moist diets. Asterisk’s ( ? ) above gray horizontal square bracket indicate significant differences between adjacent segments (glmmPQL test in R). ??? p < 0.001, ?? p < 0.01, ? p < 0.05. No significant differences occurred in fatty acid ADCs within intestinal segments between fish fed dry and moist diets. Data are mean ± SEM, n = 6.
Nutrient Markers when you look at the Plasma
Zero mathematically significant distinctions was observed to have plasma concentrations out of cholesterol, totally free greasy acid, glucose, triacylglycerides otherwise healthy protein anywhere between fish provided dry otherwise wet dieting (Figure nine).
Profile nine. Plasma levels of metabolites. (A) The levels off cholesterol levels, Chol; 100 % free fatty acids, FFAs; glucose, Glu; total triacylglycerides, TAG; and you can (B) overall healthy protein, Tprot in the plasma compiled of ballan wrasse given deceased or moist diet. Investigation are aplikacje randkowe hot or not indicate ± SEM (letter = 9 otherwise 12 for lifeless otherwise damp dieting, respectively).
The transcriptomic expressions of Segments 1 and 4 were analyzed in the two feeding groups. According to the DESeq2 analysis, 59 genes were differentially expressed (DE) (q < 0.1) between the two treatments in Segment 1 (Supplementary Table S1) 26 genes in Segment 4 (Supplementary Table S2). However, none of the DE genes in Segment 1 were overlapping with the DE genes of Segment 4 (Figure 10).