9-kb area, series positioning was in fact presented anywhere between ‘Queen’ and you may MR-1. All in all, fifteen unique SNP locus and you may 17 book InDel variations in ‘Queen’ was indeed understood (Extra Tables 5 and six). Based on gene anticipate annotation, these SNP and you will InDel variations were situated in MELO3C023548, MELO3C023549, MELO3C023550, MELO3C023551, MELO3C023554, and MELO3C023555. The latest InDel (3-bp installation-Chr.1:32354792) observed in MELO3C023555 and InDel (1-bp deletion-Chr.1: 32408632) noticed in MELO3C023548 brought about UTR_3_Best and you may UTR_5_Primary region variants, respectively. The brand new SNP (nucleotide changeover off G to help you T-Chr.1: 32392799) and you may InDel (8 bp deletion-Chr.1: 32393077) was understood from the upstream regarding MELO3C023550. One or two InDels (1-bp removal) was in fact found in the fresh upstream MELO3C023554.
Really differences, along with 14 SNPs and you may eleven InDel differences were used in MELO3C023549, and therefore the new MELO3C023549 when you look at the ‘Queen’ could be distinctive from that of almost every other melon contours. MELO3C023549 try predicted so you can encode a person in transcription foundation MYB like an MYB46-including transcription component that appeared as if linked to self-confident control out of additional phone wall structure biogenesis (Supplemental Table 4). That InDel (33 bp DNA insertion-Chr.1: 32382245) was observed regarding the intron out-of MELO3C023551. About DHL92 reference genome, so it gene is 6244 bp long that have fourteen exons. This 33-bp insertion regarding ‘Queen’ are found in 105-bp intron area ranging from exon 10 and exon 11 (scaffold00060: step one,260,2step one5–step one,260,320). MELO3C023551 was annotated to help you encode CESA catalytic subunit step 3 (UDP building) and you may regarding cellulose biosynthetic processes. Hence, we inferred you to MELO3C023549 and you can MELO3C023551 have been probably associated with wave leggere questo post qui seeds and rigid-placenta mutation within the melon ‘Queen’.
Discussion
We before discovered an interesting sensation that melon ‘Queen’ bears rigorous-placenta fresh fruit and revolution seed. Hereditary research revealed that both of these characteristics was basically controlled by the newest recessive genes ws and you may tp, correspondingly. While doing so, new cosegregation costs indicated that the fresh new rigid-placenta feature is related to the latest wave seed characteristic.
All in all, 449 SSR plified polymorphism straps that have been obvious, reproducible, and you can particular. Fifteen polymorphic SSR plify clear and you will certain polymorphism rings. The brand new polymorphism top was 22.7% (102 away from 449) anywhere between ‘Queen’ and MR-one in this study. However, it polymorphic ratio didn’t represent this new polymorphism number of SSR indicators ranging from ‘Queen’ and you can MR-1 centered on all of our most other degree. MR-step 1 and you can ‘Queen’ was basically as well as put given that mothers so you’re able to map QTL having downy mold resistance (studies unpublished). Because studies, 537 SSR primers were tested and you will 168 (30.3%) was polymorphic between MR-1 and you will ‘Queen’. It polymorphic proportion was similar to you to definitely discover ranging from AR 5 and Harukei step three (29.8%) (Fukino et al., 2008), otherwise polymorphism ratio between Edisto 47 and ‘Queen’ (30.5%) (Ning mais aussi al., 2014a). But it polymorphism peak was still lower than one located between the parents regularly derive the newest PI_PS map (44.6%) (Gonzalo et al., 2005). The low polymorphism ranging from ‘Queen’ and you can MR-step one would be associated with their way more comparable hereditary record.
Previous studies showed that the morphology traits of Cucurbitaceae seeds were quantitative traits generally controlled by multiple genes. Several QTLs associated with seed traits were detected on seven linkage groups in watermelon (Chi et al., 2017; Liu et al., 2014; Yi et al., 2014). QTL analysis of cucumber seed was concentrated on the five linkage groups (Chen et al., 2012; Wang et al., 2014). By using an F2 population developed from melon Ano2 and K413, Wang et al. (2011) found that several QTLs on LG 2, 5, 7, 8, 9, and 10 controlled SL, SD, and SW. Six QTLs for seed-related traits were detected on LG 1, 2, 5, and 8 in melon MR-1 and M1-15 (Ye et al., 2017). In this study, one major SL QTL and one major SS QTL, both linked with wave seed trait, were detected at LOD scores of 15.6 and 13.4, respectively. This result showed that wave seed shape had a significant impact on SL and SS traits during melon ‘Queen’ fruit development.